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Forensic DNA typing [electronic resource] : biology, technology, and genetics of STR markers / John M. Butler.

By: Butler, John M. (John Marshall), 1969-.
Material type: TextTextPublisher: Amsterdam ; Boston : Elsevier Academic Press, c2005Edition: 2nd ed.Description: 1 online resource (xvii, 660 p.) : ill. (some col.).ISBN: 9780080470610 (electronic bk.); 0080470610 (electronic bk.); 9780121479527 (print); 0121479528 (print).Subject(s): DNA fingerprinting | Forensic genetics | DNA -- Synthesis | DNA Fingerprinting -- methods | Databases, Nucleic Acid | Forensic Medicine | SCIENCE -- Life Sciences -- Genetics & Genomics | Gen�eticaGenre/Form: Electronic books.Additional physical formats: Print version:: Forensic DNA typing.DDC classification: 572.86 Online resources: EBSCOhost
Contents:
Overview and history of DNA typing -- DNA biology review -- Sample collection, DNA extraction and DNA quantitation -- The polymerase chain reaction (DNA amplification) -- Commonly used short tandem repeat markers and commercial kits -- Biology of STRs : stutter products, non-template addition, microvariants, null alleles, and mutation rates -- Forensic issues : degraded DNA, PCR inhibition, contamination, mixed samples and low copy number -- Single nucleotide polymorphisms and other bi-allelic markers -- Y chromosome DNA testing -- Mitochondrial DNA analysis -- Non-human DNA testing and microbial forensics -- DNA separation methods : slab-gel and capillary electrophoresis -- DNA detection methods : fluorescent dyes and silver-staining -- Instrumentation for STR typing : ABI 310, ABI 3100, FMBIO systems -- STR genotyping issues -- Laboratory validation -- New technologies, automation, and expert systems -- Combined DNA index system (CODIS) and the use of DNA databases -- Basic genetic principles, statistics, and probability -- STR population database analyses -- Profile frequency estimates, likelihood ratios, and source attribution -- Approaches to statistical analysis of mixtures and degraded DNA -- Kinship and parentage testing -- Mass disaster DNA victim identification.
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Includes bibliographical references and indexes.

Overview and history of DNA typing -- DNA biology review -- Sample collection, DNA extraction and DNA quantitation -- The polymerase chain reaction (DNA amplification) -- Commonly used short tandem repeat markers and commercial kits -- Biology of STRs : stutter products, non-template addition, microvariants, null alleles, and mutation rates -- Forensic issues : degraded DNA, PCR inhibition, contamination, mixed samples and low copy number -- Single nucleotide polymorphisms and other bi-allelic markers -- Y chromosome DNA testing -- Mitochondrial DNA analysis -- Non-human DNA testing and microbial forensics -- DNA separation methods : slab-gel and capillary electrophoresis -- DNA detection methods : fluorescent dyes and silver-staining -- Instrumentation for STR typing : ABI 310, ABI 3100, FMBIO systems -- STR genotyping issues -- Laboratory validation -- New technologies, automation, and expert systems -- Combined DNA index system (CODIS) and the use of DNA databases -- Basic genetic principles, statistics, and probability -- STR population database analyses -- Profile frequency estimates, likelihood ratios, and source attribution -- Approaches to statistical analysis of mixtures and degraded DNA -- Kinship and parentage testing -- Mass disaster DNA victim identification.

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Forensic DNA typing by Butler, John M. ©2005
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